High View Innovation (hVI)

LACTOSE (C12H22O11), also called milk sugar, is a disaccharide that consists of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. The assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The fluorescence intensity at 590nm when excited by 530nm is directly proportional to the lactose concentration in the sample measured by our fluorometer.

Samples: Serum, plasma, and other biological samples.

➥ Assay Performance:
● Sufficient for about 100 tests.
● Linear Detection Range: 0.04 to 1 mM.
● Detection Limit: 0.04 mM (1.4 mg/dL, 14 ppm).
● Typical Precision (CV%): <5%.

➥ Shipping and storage: the kit is shipped on ice. Store Assay Buffer at 4°C and other reagents at -20°C. Shelf life of 12 months after receipt.

➠ See the detailed information: Lactose Quantification with Fluorometer 530/590nm