Glycerol Quantification Assay
GLYCEROL [GLYCERIN or GLYCERINE, C3H5(OH)3] is widely used in foods, beverages and pharma-ceutical formulations. It is also a main byproduct of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications. The glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The fluorescence intensity at ex/em = 530/590nm is directly proportional to glycerol concentration in the sample measured by fluorometer.
➥ Samples: Serum, plasma, and other biological samples.
➥ Assay Performance:
● Sensitive and accurate. Use as little as 10 μL samples.
● Linear detection range: 2 -100 μM, 18 - 920 μg/dL, or 0.18 - 9.20 ppm.
● Simple and convenient. The procedure involves addition of a single working reagent and incubation for
20 min at room temperature.
●
Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal
stability.
➥ Product Information:
● Glycerol Assay Kit: Sufficient for approximately 200 assays. Kit content: (1) 24 mL
Assay Buffer; (2) 500 μL Enzyme Mix; (3) 250 μL ATP; (4) 220 μL Dye Reagent; (5) 100 μL Glycerol
Standard (100 mM).
● Mini glass tubes (optional).
● The fluorometer comes with a 5VDC power adapter, a USB
cable, manual and data management software CD.
*Avoid contact and inhalation. Standard laboratory safety procedures should be followed when handling
this product. Safety procedures include wearing OSHA approved safety glasses, gloves and protective
clothing.
*Shipping and storage: the kit is shipped on ice. Store Assay Buffer at 4°C and other reagents at -20°C
➠ See the detailed information: Glycerol Quantification with Fluorometer 530/590nm