Acetate Quantification Kit
ACETATE is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar, and extensively used in food, dyes, paints, glue and synthetic fibres. The assay uses enzyme-coupled reactions to form a colored, fluorescent product. The fluorescence intensity, measured using our handheld fluorometer (ex/em = 530/590nm), is directly proportional to acetate concentration in the sample.
➥ Assay Performance:
● Sensitive and accurate. Use as little as 10 μL samples.
● Linear detection range: 0.1 – 1.0 mM.
➥ Field Kit includes:
●Handheld Fluorometer, with USB Cable, 5VDC Power Supply, and manual/data-management software CD.
●Acetate Assay Kit: Sufficient for approximately 100 assays. Kit content: (1) 25 mL Assay Buffer, (2) Enzyme A (dried), (3) Enzyme B (dried), (4) 120 μL ATP, (5) 120 μL Dye Reagent, (6) 1 mL Developer, (7) 1 mL Standard.
●Mini glass tubes (optional).
※NOTE:
*Avoid contact and inhalation. Standard laboratory safety procedures should be followed when handling this product. Safety procedures include wearing OSHA approved safety glasses, gloves and protective clothing.
*Shipping and storage: the kit is shipped on ice. Store all reagents at -20°C. Shelf life of 12 months after receipt.
➠ See the detailed information: Acetate Quantification with Fluorometer 530/590nm