High View Innovation (hVI)

dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts. hVI offers three dsDNA quantitiation kits, including Broad Range, High Sensitivity and Ultra High Sensitivity. The kits also differ in their tolerance of various contaminants. The Ultra High Sensitivity assay is based on a novel, next-generation DNA binding dye which offers unrivaled sensitivity and dynamic range compared to other DNA dyes.

➥ Broad Range
● Linear range: 2-2000 ng dsDNA. 1500 assays.
● Blue fluorescence (Ex/Em: 350/460 nm)
● Assay can be extended to 4000 ng dsDNA with minor loss of linearity
● Blue fluorescence detection, compatible with handheld fluorometer (360nm)

➥ High Sensitivity
● Linear range: 0.2-100 ng dsDNA. 1000 assays.
● Green fluorescence (Ex/Em: 480/530 nm)
● Membrane-impermeable dye is non-toxic and non-mutagenic, for safer handling and easy disposal
● Green fluorescence detection, compatible with handheld fluorometer (480/530 nm)

➥ Ultra High Sensitivity
● Linear range: 0.03-250 ng dsDNA. 1000 assays.
● Green fluorescence (Ex/Em: 480/530 nm)
● Unrivaled sensitivity and dynamic range
● Novel green fluorescent dye is a perfect match for blue LED excitation sources
● Green fluorescence detection, compatible with handheld fluorometer (480/530 nm)

Four easy steps to measure dsDNA samples:
Step 1: Prepare quantitation working solution
Step 2: Add working solution
Step 3: Add standards or samples
Step 4: Measure fluorescence

※ All kits are stored in 4℃, and the shelf life is 12 months if properly stored.